Super Critical Liquid Chromatography

Super Critical Liquid Chromatography

SUPER CRITICAL FLUID CHROMATOGRAPHY BY ABIJAH 08171S0417 MPAQA SUPER CRITICAL LIQUID CHROMATOGRAPHY Def Introduction

Principle Instrumentation Applications Definition Chromatography: It is a method of separation of complex mixtures .

Super critical liquid chromatography: It is a type of column chromatography in which super critical fluid is used as a mobile Phase for the separation of mixtures. Super critical fluid: A super critical fluid is that which contains temperature and pressure above its critical temperature and pressure. The

critical temperature of a substance is the temperature above which a distinct liquid phase cannot exist regardless of pressure. The critical pressure is that the vapour pressure at its critical temperature. No matter how much temperature and pressure is applied the substance cannot exists as a liquid. Introduction

This technique is a hybrid of Gas and High performance liquid chromatography. It is relatively a recent chromatographic technique which is commercially available since 1982.

Advantages such as rapid separation without the use of organic solvents, faster separation than HPLC, high resolution chromatography than GC thereby faster analysis of thermo labile compounds. Super critical temperature and pressure are the defining boundaries for a pure substance. Beyond these boundaries the properties of a

substances are intermediate between a liquid and gas. In this region the fluid has a good solvating power and high diffusivity. Instrumentation

An HPLC like setup with two reciprocating pumps designed to provide a mixed mobile phase with a packed analytical column placed in an oven followed by an optical detector in which the pressure and flow rates can be independently controlled . A GC like setup with a syringe pump followed by a capillary column in a GC oven with a restrictor followed by a flame ionization detector, where the pressure is controlled by the flow rate of the pump.

In SFC the mobile phase is initially pumped as a liquid and is brought into the super critical region by heating it above super critical temperature before it enters the analytical column.

It passes through an injection valve where the sample is introduced into the super critical stream and then into the analytical column. It is maintained supercritical as it passes through the column and into the detector by a pressure restrictor placed either after the detector or at the end of the column.

Restrictor prevents clogging Mobile phase: Carbon dioxide, ethane, butane, nitrous oxide, dichlorodifluoromethane, diethyl ether, ammonia and tetrahydrofuran. Reciprocating pumps Syringe pumps

LC Injection valve (Pneumatically driven valves) Conventional GC or LC ovens Capillary, stainless, Open tubular columns. Stationary phase: Fused silica Detectors: Optical, Flame ionization detector, Spectroscopic detectors.

The results of a run in SFC are a chromatogram. Example: Chromatogram of separation of polymers. It is schematically shown as follows. Applications Purification of thermo labile compounds.

Characterization of polymers. Complex distributions can be resolved, low temperatures can be used Oligomers can be detected. Solute diffusivities Separation of enantiomers Metabolites of estrogens are separated by

SFC . Separation of chiral and Achiral mixtures. Natural products, drugs, foods, Pesticides and herbicides, surfactants, fossil and propellants.

Recently Viewed Presentations

  • acl engineering a/s advanced well engineering delivered presentation

    acl engineering a/s advanced well engineering delivered presentation

    presentation to running order ACL Engineering Services Areas of Specialism Strategic Partners Tools and Methodologies A Recent Case Study advanced well engineering…delivered ACL Engineering A/S is an independent company that focuses on the management, engineering and delivery of complex well...
  • Poetry Anthology - My CPA

    Poetry Anthology - My CPA

    Warren Pryor. Annotating (If you got less than 4): Figurative and Literal Underlining has to be clearer (No Waves) When identifying poetic devices ensure that it is accurate and that you complete T-charts for metaphors. Identify and Explode .
  • La Saga Simca - arppaulstra

    La Saga Simca - arppaulstra

    La Saga Simca La Saga Matra - Simca Le Rancho v6
  • Comparison of Student Learning in Challenge-based and Traditional

    Comparison of Student Learning in Challenge-based and Traditional

    Comparison of Student Learning in Challenge-based and Traditional Instruction in Biomedical Engineering Others: Taylor Martin , Stephanie D. Rivale, and Kenneth R. Diller
  • Cells - Home - Trousdale County Schools

    Cells - Home - Trousdale County Schools

    Microscopy and Cheek Cells. Biology, 9th ed,Sylvia Mader. Cell Structure and Function. Slide # Chapter 04. Cell Size. Most much smaller than one millimeter (mm) Some as small as one micrometer (mm) Size restricted by Surface/Volume (S/V) ratio.
  • Client-Based Access Control Management for XML documents Luc ...

    Client-Based Access Control Management for XML documents Luc ...

    Random Writes - Samsung SSD Out of the box Random Writes - Samsung SSD Out of the box Random Writes - Samsung SSD After filling the device Startup and running phases for the Mtron SSD (RW) Running phase for the...
  • 3rd Grade Journeys - Central Dauphin School District

    3rd Grade Journeys - Central Dauphin School District

    My Brother Martin: A Sister Remembers Growing Up with the Rev. Dr. Martin Luther King Jr. by Christine King Farris. How Tia Lola Came to Stay . by Julia Alvarez. The Power of W.O.W!. by Crystal Hubbard. Stormalong. by Mary...
  • Guide Word Fun! - North Allegheny

    Guide Word Fun! - North Allegheny

    Guide Words. Guide Wordsare the words at the top of each dictionary page that help you to know what words can be found on that page. They are incredibly helpful when you are looking for a certain word in the...