Spectroscopy and its Application

Spectroscopy and its Application

Spectroscopy and its Application Chemical and Biological detection Professor: Nam Sun Wang Haimo Liu 12/04/2007 Spectrum and Spectroscopy Spectrum: (a). Different colors observed when

the white light was dispersed through the prism (b). The changing of light intensity as a function of frequency Spectroscopy: Study of spectrum, to identify substances Spectroscopy

Types of spectroscopy: (a) Continuous spectroscopy (b) Absorption spectroscopy (c) Emission spectroscopy Emission Process Electrons ground level Energy emission Absorb energy

High energy level Equipment Left: Equipment diagram Right: Schematic diagram Applications

Absorption spectrum: used in deducing the presence of elements in stars and other gaseous objects which cannot be measured directly. Emission spectrum: provide a definition of the spectrum of each atom, used to be compared with absorption spectrum

Spectrum of planets Compare the absorption spectrum with the elements emission spectrum, people can build the spectrum of

planets. Fluorescence Spectroscopy Light source, self-emission which means the electrons transferred to the lowest level spontaneously Different fluorescence: (a) different meta-stable

states (b) different various vibrational states of the ground state Time-resolved fluorescence spectroscopy It provides fluorescence intensity

decay in terms of lifetimes Advantages: enhance the discrimination among fluorophores (overlapping emission spectra ) sensitive to various parameters of the biological microenvironment Time-resolved fluorescence spectroscopy

Time-resolved laser-induced fluorescence spectroscopy (tr-LIFS) Mathematical method y[n] T [ x[n]] T [ x[n] y[n]] T [ x[n]] T [ y[n]] T [ x[n]] T [ x[n]] x[n] x[k ] [n k ] k

T [ x[n]] T [ x[k ] [n k ] k Mathematical method T [ x[n]] x[k ]T [ [n k ]] k y[n] x[k ]T [ [n k ]] k

If [ x] is an impulse, then the impulse response will beh[ x ] T [ [ x]] h[n k ] T [ [n k ]] y[n] x[k ]h[n k ] k Mathematical method

Based on the definition of convolution: ( f g )(m) f (n) g (m n) n y[n] ( x h)[n] x[n] h[n] Mathematical method For the tr-LIFS system, the impulse (t )

responseIRF function is what would be recorded as the observed fluorescence F (t ) decay F (t ) I (t ) IRF (t ) Estimation of the intrinsic

fluorescence decay was carried out via deconvolution of the observed fluorescence Spectral Imaging system Imaging provides intensity at every pixel of the image I (x, y)

spectrometer provides the intensity of a single spectrum, I() spectral image provides a spectrum at each pixel, I (x, y, )

Observation of multiple activities Trying to use 5 different kinds of fluorescent molecules to label each of the 24 chromosomes in human body 2 to 5 minus 1=31 Observation of multiple colors

Living cell spectral imaging Compromise: only two kinds of cellular organ were labeled Questions?

Recently Viewed Presentations

  • What I Learned About Assessment From the AP

    What I Learned About Assessment From the AP

    What I Learned About Assessment From the AP Program Dan Kennedy Baylor School Houston AP Teachers Meeting November 11, 2006 AP Calculus Grading Rubrics If the AP readers can give partial credit fairly to 250,000 students, I ought to be...
  • O Que  o SciFinder?   uma ferramenta do CAS

    O Que o SciFinder? uma ferramenta do CAS

    Resultados - Refine Ferramenta de refino de uma lista de resultados Resultados - Explore Substances Ferramenta de análise de uma lista de resultados Resultados - Export É possível exportar uma lista de resultados em diversos formatos Resultados - Keep Me...
  • A kite time line. - whkf.org.uk

    A kite time line. - whkf.org.uk

    A nice little story is that the first kite was from a Chinaman who was out fishing and the wind blew his coolie hat off. As he cast his line out the hook caught on the brim and it sailed...
  • The Parapsychology of the Horse-Human Relationship

    The Parapsychology of the Horse-Human Relationship

    The Parapsychology of the Horse-Human Relationship Beverley Kane, MD Stanford Center for Research in Disease Prevention San Francisco Parapsychology Research Group Manual of Medicine & Horsemanship Core Message The most powerful and transformative phenomena in the horse-human relationship and in...
  • The Policy Oscars: In Search of Best Practice

    The Policy Oscars: In Search of Best Practice

    "University rules, policies and guidelines tend to focus on the punitive aspects of procedures rather than the educative and often fail to articulate to students why the conventions of citing and referencing are an integral part of developing scholarly writing...
  • People Admin User Preview Session - PPT DECK v4.1

    People Admin User Preview Session - PPT DECK v4.1

    Step 12 Employment & Compensation Team Elaine Peters - Employment & Compensation Director Anders Askenas - Employment Services Consultant Lucie Dean - Compensation Services Consultant Ginny Flenoy - Employment Services Consultant Ron Guizado - Employment Services Consultant Gwen Kobayashi -...
  • Thermal modeling of Nb3Sn Superconducting Magnets

    Thermal modeling of Nb3Sn Superconducting Magnets

    Material data collection and compilation. Temperature range of 1.0K to 300.0K. The compiled dataset will be used as input to the Network model for Nb 3. Sn Superconducting Magnets.. Assess the impact of material properties on thermal properties of magnets.
  • Introduction to solid mechanics - Olin College

    Introduction to solid mechanics - Olin College

    This relation is easy to prove by geometry Neutral axis Normal stress in bending M Take a slice through the beam σ Neutral axis is the centroid y Flexure formula Will derive this in Mechanics of Solids and Structures b...