Development of Polymeric and Liposomal Nanohybrids as Potential

Development of Polymeric and Liposomal Nanohybrids as Potential

Development of Polymeric and Liposomal Nanohybrids as Potential
Targeting Drug Delivery Systems
Jeromy T. Bentley, RET Fellow 2010
Naperville Central High School
RET Mentor: Dr. Seungpyo Hong, PhD
NSF- RET Program

Introduction

Abstract

This year, an estimated 569,490 Americans are expected to die of cancer, more than
1,500 people a day. An estimated 1,529,560 new cases of cancer will be diagnosed in the
U.S. in 2010.1 Cancer is the second most common cause of death in the US, exceeded
only by heart disease. In the US, cancer accounts for nearly 1 of every 4 deaths. Most of
the currently available chemotherapy treatments frequently accompany severe side effects
due to high toxicity to normal cells and tissues, thus targeting tumor cells and tissues is a
worthwhile endeavor. Passive targeting utilizes the enhanced permeability and retention
(EPR) effect that is defined by leaky vasculature around tumors, resulting in the
accumulation of the nanoscale delivery system at the tumor site.2 In order to take
advantage of the EPR effect, a nanoscale delivery system needs to be in the range of 50200 nm.
The objective of the study was to encapsulate functionalized polyethyleneimine (PEI) into
polymeric nanoparticles or liposomes at a controlled size range less than 200 nm. Two
types of novel nanohybrid systems have been prepared that encapsulate PEI-Rhodamine
(PEI-RHO) conjugates, as outlined in the hypothesis below.

Hypothesis

Polycations (positively charged polymers) have demonstrated potential
as nonviral drug delivery systems, however high toxicity has hindered
their applications in vivo. This is most likely due to the cationic surface
that induces uncontrolled, spontaneous interactions with cells.3 For this
reason, control over the cellular interactions of a potential drug delivery
system would be beneficial. Two nanohybrid systems (100-150 nm in
diameter) that combine the polycations with protective outer layers
consisting of biodegradable polymers or liposomes were synthesized.
Polyethylenimine (PEI), a common polycation, was employed after
conjugation with rhodamine (RHO). Encapsulation of the PEI-RHO
conjugates into: i) polymers of polyethylene oxide-b-polylactide (PEOPLA) or ii) PEGylated anionic liposomes, resulted in the production of
two new nanohybrid systems. The results present a potential drug
delivery system with enhanced control over its biological interactions
and passive targeting potential through size control.

Materials and Methods

Results
.

Conclusion/Future
Studies

Based on particle size and surface charge characterization
studies, both nanoparticles have successfully been synthesized.
Successful conjugation of various pharmaceuticals with PEI and
subsequent encapsulation can be studied in the future.
Successful passive targeting of cancer cells can also be studied
in the future through cellular uptake and invitro studies.

Teaching Module
Plan

Who: Naperville Central students and Dr. Hongs
Lab
What: Mini-Conferences covering Polymer and
Chemistry and Nanoparticle synthesis
Where: University of Illinois-Chicago

Acknowledgemen
ts

NSF Grant # EEC-0743068

Dr. Andreas Linninger, RET Program Director
Dr. Seungpyo Hong, Faculty Research Mentor
Suhair Sunoqrot, Graduate Research Mentor

When: Summer 2011

Undergraduate/ Graduate staff in Dr. Hongs
Lab

Why: Exposure of cutting edge research inspires
students to study science in the future

University of Illinois- Chicago

1. American Cancer Society. Cancer Facts & Figures 2010. Atlanta: American Cancer Society; 2010.
2. Peer et al., Nanocarriers as an emerging platform for cancer therapy. Nature Nanotech. 2007, 2, 751-60.
3. Verma, I. M.; Somia, N., Gene therapy - promises, problems and prospects. Nature 1997, 389 (6648), 239-242.

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