Components Pumps 1000 - 6000 psi at rate of @ 3 mL/min reproducible to 2% screw driven syringe
reciprocating pump A reciprocating pump for HPLC.
Components Precolumns remove impurities from solvent saturates mobile phase with liquid of stationary phase before the analytical column
Components Sample Injection System sample valve syringe
A sampling loop for liquid chromatography Components http://www.youtube.com/watch?v=mPO7Tv2mIJU
Columns straight, 15 to 150 cm in length; 2 to 3 mm i.d. packing - silica gel, alumina, Celite
Normal phase Polar Stationary Phase and Nonpolar Solvent Reverse phase Nonpolar Stationary Phase and Polar Solvent Size exclusion (gel permeation) - Small molecules penetrate into the pores within the packing while larger molecules only partially penetrate the pores. The large molecules elute before
the smaller molecules. Effect of Particle Size Columns Cont.
Components Temperature Control Components
Detectors single wavelength UV tuneable UV diode-array refractive index
MS Performances of LC Detectors Ultraviolet
detector cell for HPLC Diode-Array Absorption
Spectra Absorption spectra of the eluent from a mixture of
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