CHEMISTRY IN C.S.I. CRIME SCENE INVESTIGATIO IS THE BLOOD ALWAYS RED? MARTINA STANKOVA 1B GROUP 57 FACULTY OF CHEMICAL AND FOOD TECHNOLOGY SUMMARY 1.Haemoglobin - the unique compound of blood 2.Techniques of bloods detection Luminescene Crystal reaction Catalytic color reaction Instrumental reaction 3.Resume of detection techniques MARTINA STANKOVA, 1B GROUP 57, FACULTY OF CHEMICAL AND FOOD TECHNOLOGY, SLOVAK UNIVERSITY OF TECHNOLOGY CHEMISTRY IN C.S.I. IS THE BLOOD ALWAYS RED? 2 HAEMOGLOBIN protein's subunits protein's subunits iron-containing heme groups Structure of human haemoglobin. MARTINA STANKOVA, 1B GROUP 57, FACULTY OF CHEMICAL AND FOOD TECHNOLOGY, SLOVAK UNIVERSITY OF
TECHNOLOGY CHEMISTRY IN C.S.I. IS THE BLOOD ALWAYS RED? 3 TECHNIQUES OF BLOODS DETECTION Techniq ue based on Heme stain Protein s stain MARTINA STANKOVA, 1B GROUP 57, FACULTY OF CHEMICAL AND FOOD TECHNOLOGY, SLOVAK UNIVERSITY OF TECHNOLOGY Luminescence Luminol/ Lumiscene Fluorescein Crystal test Takayama test Catalytic color test Leucocrystal violet Kastle-Meyer test Instrumental
test High performance liquid chromatography Catalytic test Amido black CHEMISTRY IN C.S.I. IS THE BLOOD ALWAYS RED? 4 CHEMILUMINESCENCE OF LUMINOL + h 425 nm 1 MARTINA STANKOVA, 1B GROUP 57, FACULTY OF CHEMICAL AND FOOD TECHNOLOGY, SLOVAK UNIVERSITY OF TECHNOLOGY CHEMISTRY IN C.S.I. IS THE BLOOD ALWAYS RED? 5 LUMINOL EMISSION SPECTRUM Luminol emission spectrum : peak at 425 nm Human eye range : from 380 nm to 780 nm MARTINA STANKOVA, 1B GROUP 57, FACULTY OF CHEMICAL AND FOOD TECHNOLOGY, SLOVAK UNIVERSITY OF TECHNOLOGY Detection of blood using Luminol on
crime scene CHEMISTRY IN C.S.I. IS THE BLOOD ALWAYS RED? 6 LUMISCENE IMPROVED LUMINOL Lumiscenes emisson spectrum peak at 525 nm Color corresponding to wavelenght of 525 nm Same reaction but better wavelenght and eye sensitivity MARTINA STANKOVA, 1B GROUP 57, FACULTY OF CHEMICAL AND FOOD TECHNOLOGY, SLOVAK UNIVERSITY OF TECHNOLOGY CHEMISTRY IN C.S.I. IS THE BLOOD ALWAYS RED? 7 KASTLE-MEYER TEST PHENOLPHTHALIN MARTINA STANKOVA, 1B GROUP 57, FACULTY OF CHEMICAL AND FOOD TECHNOLOGY, SLOVAK UNIVERSITY OF TECHNOLOGY CHEMISTRY IN C.S.I. IS THE BLOOD ALWAYS RED? 8 NEGATIVE/POSITIVE KASTLE-MEYER TEST Negative and positive result to presence of blood
MARTINA STANKOVA, 1B GROUP 57, FACULTY OF CHEMICAL AND FOOD TECHNOLOGY, SLOVAK UNIVERSITY OF TECHNOLOGY CHEMISTRY IN C.S.I. IS THE BLOOD ALWAYS RED? 9 RESUME Compound Use Color Sensitivity Persistance Interference Damage Luminol Reagent for latent bloodstains Blue glow 0,0001 ml of blood Photographs should be taken within a few hours Copper salts, brass, bronze and other similar alloys No damage KastleMeyer test
Reagent for latent and visible bloodstain Pink 0,000 001 ml of blood Immediate change of color Vegetable peroxidases, copper, nickel salts Damage of DNA LCV Latent prints, deposited blood on non-porous surfaces,visible blood prints Purple 0,001 ml of blood Immediate change of color Copper, nickel and other similar salts Damage of DNA Takayama test Reagent for latent bloodstain
Pink crystals 0,001 ml of blood Formation of crystals none Damage of DNA Fluorescein Reagent for latent bloodstains Green fluorescence 0,0001 ml of blood Photographs should be taken within a few hours Copper, nickel and other similar salts No damage Amido black Latent prints and to enhance visible blood prints on painted surfaces Dark blue
0,001 ml of blood Photographs should be taken within a few hours MARTINA STANKOVA, 1B GROUP 57, FACULTY OF CHEMICAL AND FOOD TECHNOLOGY, SLOVAK UNIVERSITY OF TECHNOLOGY CHEMISTRY IN C.S.I. IS THE BLOOD ALWAYS RED? Damage of blood sequence 10 Compound Use Color Sensitivity Persistance Interference Damage Luminol Reagent for latent bloodstains Blue glow 0,0001 ml of blood Photographs should
be taken within a few hours Copper salts, brass, bronze and other similar alloys No damage KastleMeyer test Reagent for latent bloodstain Pink 0,000 001 ml of blood Immediate change of color Vegetable peroxidases, copper, nickel salts Damage of DNA LCV Latent prints, deposited blood on non-porous surfaces,visible blood prints Purple 0,001 ml of blood Immediate change of color Copper, nickel and
other similar salts Damage of DNA Takayama test Reagent for latent bloodstain Pink crystals 0,001 ml of blood Formation of crystals None Damage of DNA Fluorescein Reagent for latent bloodstains Copper, nickel and other similar salts No damage Amido black Latent prints and to enhance visible blood prints on painted surfaces THANK YOU ATTENTION
FOR QUESTIONS? Green 0,0001 ml of Photographs should fluorescence blood be taken within a few hours Dark blue 0,001 ml of blood Photographs should be taken within a few hours MARTINA STANKOVA, 1B GROUP 57, FACULTY OF CHEMICAL AND FOOD TECHNOLOGY, SLOVAK UNIVERSITY OF TECHNOLOGY YOUR CHEMISTRY IN C.S.I. IS THE BLOOD ALWAYS RED? Damage of DNA 11 REFERENCES Dr R V Winchester, XII-Biotech-A-Blood Detection-6;
O. Germain, C. Fregeau, and R.M. Fourney, Evaluation of the Effects of Blood Enhancement Chemicals on the Subsequent Short Tandem Repeat DNA Typing Analysis of Blood Fingerprints; Shannon M. Casey, Hilary M. Stinnett, Gory Dactyloscopy; http://www.evidentcrimescene.com/cata/chem/chem.html http://www.lumiscene.com/indexa.html http:// www.sciencebuddies.org/science-fair-projects/project_ideas/BioChe m_p037.shtml http://www.inreferencetomurder.com/html/crime_scene.html MARTINA STANKOVA, 1B GROUP 57, FACULTY OF CHEMICAL AND FOOD TECHNOLOGY, SLOVAK UNIVERSITY OF TECHNOLOGY CHEMISTRY IN C.S.I. IS THE BLOOD ALWAYS RED? 12
