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PiXL Club The PiXL Club The PiXL Club The PiXL Club Th such time it may be freely used within the member school. PiXL Club The PiXL Club The PiXL Club The PiXL Club The PiXL Club The PiXL Club The PiXL Club The PiXL Club The PiXL Club The PiXL Club The PiXL Club The PiXL Club The PiXL Club The PiXL Club The P Club The PiXL Club The PiXL Club The PiXL Club The PiXL Club The PiXL Club The PiXL Club The PiXL Club The PiXL Club The PiXL Club The PiXL Club The PiXL Club The PiXL Club The PiXL Club The PiXL C All opinions and contributions are those of the authors. The contents of this resource are not connected with The PiXL Club The PiXL Club The PiXL Club The PiXL Club The PiXL Club The PiXL Club The PiXL Club The PiXL Club The PiXL Club The PiXL Club The PiXL Club The PiXL Club The PiXL Club The PiXL Club Th by The anyPiXL other organisation institution. PiXL Club The PiXL Club The PiXLnor Clubendorsed The PiXL Club Clubcompany,

The PiXL Club The PiXL CluborThe PiXL Club The PiXL Club The PiXL Club The PiXL Club The PiXL Club The PiXL Club The PiXL Club The P Club The PiXL Club The PiXL Club The PiXL Club The PiXL Club The PiXL Club The PiXL Club The PiXL Club The PiXL Club The PiXL Club The PiXL Club The PiXL Club The PiXL Club The PiXL Club The PiXL C The PiXL Club The PiXL Club The PiXL Club The PiXL Club The PiXL Club The PiXL Club The PiXL Club The PiXL Club The PiXL Club The PiXL Club The PiXL Club The PiXL Club The PiXL Club The PiXL Club Th PiXL Club The PiXL Club The PiXL Club The PiXL Club The PiXL Club The PiXL Club The PiXL Club The PiXL Club The PiXL Club The PiXL Club The PiXL Club The PiXL Club The PiXL Club The PiXL Club The P Club The PiXL Club The PiXL Club The PiXL Club The PiXL Club The PiXL Club The PiXL Club The PiXL Club The PiXL Club The PiXL Club The PiXL Club The PiXL Club The PiXL Club The PiXL Club The PiXL C The PiXL Club The PiXL Club The PiXL Club The PiXL Club The PiXL Club The PiXL Club The PiXL Club The PiXL Club The PiXL Club The PiXL Club The PiXL Club The PiXL Club The PiXL Club The PiXL Club Th PiXL Club The PiXL Clubwww.pixl.org.uk The PiXL Club The PiXL Club The PiXL Club The PiXL Club TheThe PiXLPiXL ClubClub The Ltd, PiXL Company Club The PiXL Club The PiXL Club The PiXL Club The PiXL Club The PiXL Club The PiXL Club The P number 07321607

Club The PiXL Club The PiXL Club The PiXL Club The PiXL Club The PiXL Club The PiXL Club The PiXL Club The PiXL Club The PiXL Club The PiXL Club The PiXL Club The PiXL Club The PiXL Club The PiXL C The PiXL Club The PiXL Club The PiXL Club The PiXL Club The PiXL Club The PiXL Club The PiXL Club The PiXL Club The PiXL Club The PiXL Club The PiXL Club The PiXL Club The PiXL Club The PiXL Club Th Required Practical Guide Biology Microscopy This document contains: Links to YouTube clips showing the practical procedure Information from examination boards AQA, OCR, Edexcel Potential examination questions and answers Commissioned by The PiXL Club Ltd. November 2016

Copyright The PiXL Club Ltd, 2016 Required practical exam boards AQA Practical procedure the microscope http://filestore.aqa.org.uk/resources/science/AQA-8464-8465-PRACTICALS-HB.DOCX Practical procedure: Preparing a slide Onion video 1 Cheek cell video 1 Onion video 2

Cheek cell video 2 Possible examination style questions Here is a microscope image of an onion slide: 1. A student measured the length of one cells in the image as 6mm long. The magnification used was x 1500. Calculate the actual length of the cell in mm. 2. Describe how the student used a light microscope to view the cell. 3. Name a structure you would expect to see in a plant cell which is not visible in this image Possible examination style questions answers

Q1 A student measured the length of one cells in the image as 6mm long. The magnification used was x 1500. Calculate the actual length of the cell in mm. ANS: actual length= 6/ 1500 = 0.006mm Possible examination style questions answers Q2 Describe how the student used a light microscope to view the cell. ANS: Remove a single layer of cells from the onion. Place on a microscope slide and add a couple of drops of iodine.

Place a cover slip on top. or Turn the focus wheel to obtain a clear image. Start on the lowest magnification. Increase the magnification of the objective lens and refocus. Possible examination style questions answers Q3 Name a structure you would expect to see in a plant cell which is not visible in this image ANS: Vacuole Chloroplast Ribosome Mitochondria Key questions:

What sub-cellular structures are likely to be observed using a light microscope? Why are some sub-cellular structures not visible using a light microscope? What precautions are needed when preparing a microscope slide? Why should samples of animal cells be disposed of in disinfectant? Why does the layer of specimen cells need to be thin? Why do we stain the specimen cells? Why does the coverslip have to be carefully applied? What is the highest magnification for the light microscope? How is the focusing wheel used? When should the fine focusing wheel be used? What is a field of view and how can its size be measured? How is the actual size of a cell structure determined from microscope measurements? How can more detail be observed in cells? What are the important features of a drawing made from observations? http://qualifications.pearson.com/content/dam/pdf/GCSE/Science/2016/teaching-and-learning-materials/GCSE-9-1-Sciences-core-practical-guide.pdf AQA suggested examination questions

http://filestore.aqa.org.uk/resources/science/AQA-GCSE-SCIENCE-PIE.PDF AQA suggested answers http://filestore.aqa.org.uk/resources/science/AQA-GCSE-SCIENCE-PIE.PDF Edexcel suggested examination questions http://qualifications.pearson.com/content/dam/pdf/GCSE/Science/2016/teaching-and-learning-materials/GCSE-9-1-Sc iences-core-practical-guide.pdf Edexcel suggested examination answers http://qualifications.pearson.com/content/dam/pdf/GCSE/Science/2016/teaching-and-learning-materials/GCSE-9-1-Sc iences-core-practical-guide.pdf OCR suggested examination questions http://www.ocr.org.uk/Images/309171-pag-activity-biology-microscopy-suggestion-1.doc

OCR suggested answers http://www.ocr.org.uk/Images/309171-pag-activity-biology-microscopy-suggestion-1.doc Summary document A summary document is also available on Huddle which contains all the relevant information about this practical from the different examination boards. This document includes practical methods and other potential examination questions

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