Gluconeogenesis - Florida International University

Chapter 14 Glycolysis, Gluconeogenesis, and the Pentose Phosphate Pathway Part 2: Gluconeogenesis and PPP

Gluconeogenesis and PPP Learning Goals. Know: 1. Entry to gluconeogenesis.

2. Glycolysis backwards: which enzymes are at Eq and those that are the drivers. 3. What makes some amino acids glucogenic. 4. Pentose Phosphate Pathway entry by glucose-6-P

and transaldolases, transketolases. 5. Why the PPP is a web pathway. Gluconeogenesis Forms Sugars from Other Metabolites

Going Forwards and Backwards

The Beginning of Gluconeogenesis Pyruvate Carboxylase

PEP Carboxykinase Pyruvate Actually Has Two Ways to PEP

The End of Gluconeogenesis Gluconeogenesis Energetics

EOC Problem 21: overall energetics of Glycolysis and Gluconeogenesis. EOC Problem 22: Concept of a Futile Cycle.

Pentose Phosphate Pathway Beginning +aromatic amino acids + His

NADPH and Oxygen Toxicity SuperOxide Dismutase

PPP Beginning from Glucose-6-Phosphate Lactonase

6-Phosphogluconate DH Phosphopentose Isomerase (not an Epimerase)

Ribose-5-Phosphate Epimerase PPP the Web like Pathway Stuck

onto the Side of Glycolysis PPP Transketolases transfer 2 Carbon units

PPP Transketolases transfer 2 Carbon units PPP Transaldolases transfer 3 Carbon units

Another Transketolase PPP Arranged to Oxidize Glucose (6 in and 5 out)

6 Glucose-6-P Control of PPP by NADPH

PPP Functions: 1. Produce Ribose-5-P for Nucleotide Synthesis 2. Produce Ribose-5-P for His Synthesis 3. Produce Erythrose-4P for Trp, Tyr, Phe Synthesis

WYF 4. Can Completely Oxidize Glucose 5. Source of NADPH (Biosynthesis, Oxygen Killing System)

6. Can be Rearranged to Calvin Cycle Fix CO2 7. Other Specialized Reasons (Heterofermenters, etc. mainly in prokaryotes)

Things to Know and Do Before Class 1. Entry to gluconeogenesis. 2. Glycolysis backwards: which enzymes are at Eq and those that are the drivers.

3. What makes some amino acids glucogenic. 4. Pentose Phosphate Pathway entry by glucose-6-P and transaldolases, transketolases. 5. Why the PPP is a web pathway.

6. EOC Problems: 21, 22, 25 27.