Practical tips for cloning, expressing and purifying proteins
Similar to elongin, CRSP70 I II III Partial proteolysis in 96 well plates Optimized set of proteases Low protein requirement No SDS-PAGE No N-terminal sequencing Direct identification of domains by mass spectrometry DomainHunterTM 23000 28000 33000 m/z -1.0 -0.8 -0.6...