Conformational Change of the Hexagonally Packed Intermediate ...

Conformational Change of the Hexagonally Packed Intermediate ...

Conformational Change of the Hexagonally Packed Intermediate Layer of Deinococcus radiodurans Monitored by Atomic Force Microscopy Daniel J. Muller, Wolfgang Baumeister, and Andreas Engel Presented by Robert Warden Bacterial Surface Layers Protein layer surrounds cells

Usually a single protein Self-assemble into regular patterns Hydrophobic/hydrophilic sides Involved in transport two and from cell D. radiodurans S-Layer Hexagonal 6-mer Well-characterized structurally and biochemically

Atomic Force Microscopy Protein in native environment Different cantilevers Hydrophilic Si3N4 Hydrophobic Carbon

Forces of <0.2 nN Study dynamic processes AFM Images Consistent with previous studies Images of inner

surface (hydrophobic) Dynamic Studies Spontaneous open/closed shift Not effected

by change of cantilever 5 min Significance AFM can be done in native environment Forces too small to disrupt structure Proteins can be adsorbed to surface

Dynamic Information Questions?

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